Pengaruh Penambahan Ragi NKL dan Waktu Fermentasi terhadap Populasi Mikroorganisme selama Fermentasi Biji Kakao


  • Mulono Apriyanto Universitas Islam Indragiri, Indonesia
  • Priambada Priambada Instiper Jogja, Indonesia
  • Mohammad Imam Sufiyanto IAIN Madura, Indonesia



Fermentasi, Ragi NKL, S. cerevisiae, L. lactis, A. aceti


Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan ragi NKL dan waktu fermentasi terhadap perubahan warna keping biji kakao yang dihasilkan selama fermentasi. Penelitian menggunakan perlakuan pertama (A0) tanpa inokulasi (kontrol), perlakuan kedua (A1) dengan penambahan ragi NKL 2% diberikan pada awal fermentasi, perlakuan ketiga (A2) penambahan NKL 0,5% awal fermentasi kemudian ditambahkan 0,5% pada 24 jam selanjunya, dan 1% NKL ragi pada 48 jam sebagai persentase berat ragi/berat biji kakao segar. Fermentasi dilakukan selama 120 jam. Populasi mikrobia diukur menggunakan metode pour plate, perubahan warna keping biji menggunakan Cut Test. Penelitian menghasilkan populasi S. cerevisiae, L. lactis dan A. aceti tertinggi diantara ketiga perlakuan pada fermentasi 24, 48 dan 72 jam. Keping biji warna coklat (Ao), (A1) dan (A2) terbanyak pada perlakuan (A2) yaitu 97,01%.

The purpose of this study was to determine the effect of NKL yeast addition and fermentation time on discoloration to  cocoa bean ping produced during fermentation. The study used the first treatment (A0) without inoculation (control), the second treatment (A1) with the addition of 2% NKL yeast given at the beginning of fermentation, the third treatment (A2) the addition of NKL 0.5% initial fermentation then added 0.5% in the next 24 hours, and 1% NKL yeast at 48 hours as a percentage of yeast weight / weight of fresh cocoa beans. Fermentation is carried out for 120 hours. Microbial populations were measured using the pour plate method, discoloration of seed chips using the Cut Test. The study resulted in the highest populations of S. cerevisiae, L. lactis and A. aceti among the three treatments at 24, 48 and 72-hour fermentation. The most brown (Ao), (A1) and (A2) seed chips in the treatment (A2) were 97.01%.

Author Biographies

Mulono Apriyanto, Universitas Islam Indragiri, Indonesia

Program Studi Teknologi Pangan, Fakultas Pertanian, Universitas Islam Indragiri, Indonesia

Priambada Priambada, Instiper Jogja, Indonesia

Program Studi Teknik Pertanian, Fakultas Teknologi Pertanian, INSTIPER Yogya, Indonesia

Mohammad Imam Sufiyanto, IAIN Madura, Indonesia

Program Studi IPA, Fakultas Tarbiyah, IAIN Madura


Afoakwa, E. O., Budu, A. S., Mensah-Brown, H., Takrama, J. F., & Akomanyi, E. (2014). Changes in Biochemical and Physico-chemical Qualities during Drying of Pulp Preconditioned and Fermented Cocoa (Theobroma cacao) Beans. Journal of Nutritional Health & Food Science, 2(3), 1–8.

Andriani, N., Yenrina, R., & Nazir, N. (2020). Physicochemical, Fatty Acid and Sensory Profile of Cocoa Butter Produced from Fermented and Non-Fermented Cocoa Butter. Asian Journal of Applied Research for Community Development and Empowerment, 4(3), 6–14.

Aprilia, V., & Apriyanto, M. (2021). Bab I Pendahuluan.

Apriyanto, M. (2016). Fermentasi Biji Kakao Kering Terkendali Menggunakan Inokulum Mikrobia. Disertasi. Universitas Gadjah Mada.

Apriyanto, M., & Novitasari, R. (2021). During Fermentation, Microbiology and Biochemistry of the Cocoa Bean. International Journal of Food Science and Agriculture, 5(4), 688–691.

Aunillah, A., Purwanto, E. H., Wardiana, E. & Iflah, T. (2021). The effect of fermentation process, extraction methods and solvents on yield, total polyphenol, and antioxidant levels of cocoa beans. IOP Conference Series: Earth and Environmental Science, 828(1), 012038.

Beckett, S. T. (2019). The science of chocolate. Royal Society of Chemistry.

Camila D. S. V., R. M. C.-F., Muynarsk, E. de S. M., Pereira, G. V. de M., & Varize, L. D. L. and L. C. B. (2019). Biotechnological applications of nonconventional yeasts. In Yeasts in biotechnology (p. 107674).

Djaafar, T. F., Palupi, L. O., Marwati, T., Utami, T., & Rahayu E. S. (2019). Characteristic of Chocolate Candy Produced from Fermented Cocoa Bean with Lactobacillus plantarum HL-15 Starter Culture for Inhibition Growth of Mycotoxin -Producing Fungi. Journal of Agricultural Science and Technology, 9, 128–134. DOI : 10.17265/2161-6264/2019.02.006

Hartono. (2020). Kabupaten Indragiri Hilir Dalam Angka 2020. In BPS Kabupaten Indragiri Hilir.

Hartuti, S., Bintoro, N., Nugroho, J., Karyadi, W., & Pranoto, Y. (2018). Fermentasi Isothermal Biji Kakao (Theobroma cacao. L) dengan Sistem Aerasi Terkendali. Agritech, 38(4), 364–374.

Hinneh, M., E. A. M., Walle, D. de, AmeliaTzompa-Sosa, D., Winne, A. De, Simonis, J., Messens, K., Durme, J. Van, Afoakwa, E. O., Cooman, L. De, & Dewettinck, K. (2019). Pod storage with roasting: A tool to diversifying the flavor profiles of dark chocolates produced from ‘bulk’ cocoa beans? (part I: aroma profiling of chocolates). Food Research International, 119, 84–98.

Kustyawati, M. E., Setyani, S., Erna, M., & Setyani, S. (2012). Pengaruh penambahan inokulum campuran terhadap perubahan kimia dan mikrobiologi selama fermentasi coklat. Jurnal Teknologi & Industri Hasil Pertanian, 13(2), 73–84.

Kusuma, G., Komang, A., & Desak, I. (2020). Pengaruh Lama Fermentasi Terhadap Karakteristik Fermented Rice Drink Sebagai Minuman Probiotik Dengan Isolat Lactobacillus sp. F213. Jurnal Itepa, 9(2), 181 – 192.

Maigua, I. R. S. (2020). Estudio de los principales componentes químicos no volátiles asociados a la calidad del cacao “Nacional” de Ecuador, como herramienta en la certificación de origen. Doctoral dissertation,Universidad Politécnica de Cartagena.

Margono, W., Masuku, M. A., Albaar, N., & Tjokrodiningrat, T. S. (2020). The Effect of Cocoa Paste Percentage of Fermented Cocoa Beans on the Sensory Characteristic of Chocolate Bars. 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019), 227–280.

Masriany, M., Sari, A., & Devi, A.. (2020). Diversitas senyawa volatil dari berbagai jenis tanaman dan potensinya sebagai pengendali hama yang ramah lingkungan. In Prosiding Seminar Nasional Biologi, 6(1).

Misnawi, M., Tunjungsari, A. B., Febrianto, N., Adiandri, R. S., Fahrizal, F. & Fahrurrozi, F. (2017). Improvement of Small Scale Cocoa Fermentation Using Lactobacillus fermentumas Starter Culture. Pelita Perkebunan, 33(3), 203–210.

Mulato, Widyotomo, Misnawi, & Suharyanto. (2005). Petunjuk Teknis Pengolahan Produk Primer dan Sekunder Kakao. Pusat Penelitian Kopi dan Kakao Indonesi.

Parra, P., Negrete, T., Laguno, J., & Vega, N. (2018). Determination of the degree of fermentation of cocoa through different techniques of artificial vision. Innovation in Education and Inclusion : Proceedings of the 16th LACCEI International Multi-Conference for Engineering, Education and Technology, 2019.

Poveda, O. R., Pereira, L. B, Zeppa, G., & Stévigny, C. (2020). Cocoa bean shell—a by-product with nutritional properties and biofunctional potential. Nutrients, 12(4), 1123.

Putra, G. P. G., & Wartini, N. M. (2019). The Change of Characteristics and Antioxidant Activity of Cocoa Vinegar During Fermentation of Pulp Liquids by Adding Tape Yeast. American Journal of Agriculture and Forestry, 7(6), 270–276.

Putra, G. P. G., Wartini, N. M., & Luh Putu Trisna Darmayanti. (2019). Characteristics of cocoa vinegar from pulp liquids fermentation by various methods. In AIP Conference Proceedings, 2155. AIP Publishing LLC.

Racine, K. C., Wiersema, B. D., Griffin, L. E., Essenmacher, L. A., Lee, A. H., Hopfer, H., Lambert, J. D., & And, A. C. S. A. P. N. (2019). Flavanol polymerization is a superior predictor of ?-glucosidase inhibitory activity compared to flavanol or total polyphenol concentrations in cocoas prepared by variations in controlled fermentation and roasting of the same raw cocoa beans. Antioxidants, 8(12), 635.

Rahmadi, A., Yunus, Y., Ulfah, M., Candra, K. ., & Suwasono, S. (2020). Microorganism population, theobromine, antioxidant, and FTIR analysis of Samarinda cocoa bean fermented with Saccharomyces cerevisiae and Acetobacter aceti. Food Research, 4(6), 1912–1920.

Ramli, N., Omar, S. R., Jin, F. Y., & Thien, L. S. (2012). Physico-chemical properties of chocolate of Lactobacillus plantarum from fermented cocoa beans. Annals. Food Science and Technology, 13(1), 75–81.

Rojas, K., & Guevara, M. C. H.-A. A. (2021). Transformaciones bioquímicas del cacao (Theobroma cacao L.) durante un proceso de fermentación controlada. Agronomía Costarricense, 45(1), 53–65.

Santos, C. M. dos, Silva, A. B. C. e, & ... (2021). Biotechnological starter potential for cocoa fermentation from cabruca systems. Brazilian Journal of ….

Sobotta, F. (2019). Precursors of chocolate aroma – flavour profile comparisons of traditionally fermented cocoa and cocoa beans from fermentation-like incubation by means of HS-SPME-GC-MS-O. Doctoral dissertation, hschule für angewandte Wissenschaften Hamburg.

Vuyst, L. De, & Leroy, F. (2020). Functional role of yeasts, lactic acid bacteria and acetic acid bacteria in cocoa fermentation processes. FEMS Microbiology Reviews, 44(4), 432–452.




How to Cite

Apriyanto, M., Priambada, P., & Sufiyanto, M. I. . (2023). Pengaruh Penambahan Ragi NKL dan Waktu Fermentasi terhadap Populasi Mikroorganisme selama Fermentasi Biji Kakao . Open Science and Technology, 2(2), 180–189.